JCRB0626 TG1 Cell information
Cell type:general cells
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JCRB No.
JCRB0626
Cell Name
TG1
Profile
V79 derivative, 6-thioguanine-resistant
Other Name
Animal
hamster, Chinese
Strain
chinese
Genus
Cricetulus
Species
griseus
Sex
Age
Identity
Tissue for Primary Cancer
lung
Case history
Metastasis
Tissue Metastasized
Genetics
lung, V79 delivertive, 6TG resistant
Life Span
infinite
Crisis PDL
Morphology
Character
lung, V79 delivertive, 6TG resistant
Classify
mutant
Established by
Kuroki,T.
Registered by
Kuroki,T.
Regulation for Distribution
Comment
Use for metobolic cooperation assay.
Year
Medium
Eagle's minimal essential medium with 10% fetal calf serum.
Methods for Passages
Cells are treated with 0.02 % EDTA + 0.05 % trypsin
Cell Number on Passage
10^5 cells/60mm dish
Race
CO2 Conc.
5 %
Tissue Sampling
lung
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Cell No.
JCRB0626
Cell Name
TG1
LOT No.
040397
Lot Specification
distribution
Medium
Eagle's minimal essential medium with 10% fetal bovine serum (JRH Biosciences, 1B1062).
Temperature
37 C
Cell Density at Seeding
3.7 x 10^4 cells/sq.cm.
Methods for Passages
Cells harvested after treatment with 0.05% trypsin and 0.02% EDTA-PBS. Medium change every 2 days and subculture every 4 days.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
4.0 x 10^6
Viability at cell freezing (%)
98.3
Antibiotics Used
free
Passage Number
P4*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
LDH, NP and G6PD examined. Chinese hamster.
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Culture medium containing 5% DMSO.
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0626
Cell Name
TG1
LOT No.
950907
Lot Specification
distribution
Medium
Temperature
Cell Density at Seeding
Methods for Passages
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
Passage Number
PDL
Sterility: MYCOPLASMA
Sterility: BACTERIA
Sterility: FUNGI
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0626
Cell Name
TG1
LOT No.
070186
Lot Specification
distribution
Medium
MEM + 10 % FCS
Temperature
37
Cell Density at Seeding
10^5/60D
Methods for Passages
0.02 % EDTA + 0.05 % trypsin
Doubling Time
NT
Cell Number in Vial (cells/1ml)
2.3x10^6
Viability at cell freezing (%)
96.0
Antibiotics Used
free
Passage Number
P4*
PDL
Sterility: MYCOPLASMA
NT
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0626
Cell Name
LOT No.
940623
Lot Specification
distribution
Medium
MEM + 10 % FCS
Temperature
37
Cell Density at Seeding
Methods for Passages
0.02 % EDTA + 0.05 % trypsin
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
Passage Number
PDL
Sterility: MYCOPLASMA
Sterility: BACTERIA
Sterility: FUNGI
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information