JCRB0650.1 Mm2T12.P3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0650.1 | Cell Name | Mm2T12.P3 |
|---|---|---|---|
| Profile | Serum free subline from Mm2T cells. | Other Name | |
| Animal | Indian Muntjak | Strain | |
| Genus | Muntiacus | Species | muntjak |
| Sex | M | Age | 1-day-old |
| Identity | Tissue for Primary Cancer | thymus | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | The Mm2T12.P3 has different characteristics from the original strain. An addition of 2% FCS induces chromosomal averation to the Mm2T12.P3 cell line. Chromosoma digestion observed at the beginning and later chrosomosome number increases. |
| Classify | normal | Established by | Takaoka,T. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | This culture has been kept by serial subculture for long period by Takaoka,T. at serum free condition. | Year | 2004 |
| Medium | DM201 medium only. Serum free. | Methods for Passages | Cells are harvested by scraper gently and resuspend in to fresh medium. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | Tissue Sampling | thymus | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7586011 | Enhancement of adhesive property of epithelial cell line Mm2T by culture in the presence of methylated vitamin B12. Kitano Y,Takaoka T,Nakahara M,Harii K,Iwamori M Cell Struct Funct. 1995 Jun;20(3):211-21 |
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LOT Information
| Cell No. | JCRB0650.1 | Cell Name | Mm2T12.P3 |
|---|---|---|---|
| LOT No. | 08302004 | Lot Specification | distribution |
| Medium | DM201 medium. Serum free medium. | Temperature | 37 C |
| Cell Density at Seeding | 3-4 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested with scraper. Subculture every 10-14 days and medium change every 3-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 95.9 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 1 to 1 mixture of the culture medium and the FM-1 medium containing final 10% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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