JCRB0704 FM3A TK~
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0704 | Cell Name | FM3A TK~ |
|---|---|---|---|
| Profile | mammary carcinoma, thymidine kinase-deficient FM3A, (C3H/He mouse) | Other Name | FM3A TK~ |
| Animal | mouse | Strain | C3H/He |
| Genus | Mus | Species | musclus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | mammary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | mammary carcinoma, thymidine kinase deficient. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | mammary carcinoma |
| Classify | mutant | Established by | Ayusawa,D. |
| Registered by | Umeda,M. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | ES medium ({3051} ) with 2% fetal calf serum or E.MEM with 10% calf serum or fetal calf serum. | Methods for Passages | Prepare cell suspension by pipetting. |
| Cell Number on Passage | 2-3 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | mammary gland |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7105014 | Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate. Koyama H,Kodama H Cancer Res. 1982 Oct;42(10):4210-4 |
| Pubmed id:208186 | A mammalian cell mutant with temperature-sensitive thymidine kinase. Nakano MM,Sekiguchi T,Yamada MA Somatic Cell Genet. 1978 Mar;4(2):169-78 |
| Pubmed id:5920633 | Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium. Nakano N Tohoku J Exp Med. 1966 Jan 25;88(1):69-84 |
| Images |
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LOT Information
| Cell No. | JCRB0704 | Cell Name | FM3A TK~ |
|---|---|---|---|
| LOT No. | 012197 | Lot Specification | distribution |
| Medium | Temperature | ||
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | G6PD, LDH, NP. (mouse) | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |