JCRB0708 P3U1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0708 | Cell Name | P3U1 |
|---|---|---|---|
| Profile | myeloma | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | myeloma | |
| Life Span | Crisis PDL | ||
| Morphology | Character | myeloma | |
| Classify | Established by | Yelton,D.E. et al. | |
| Registered by | Umeda,M. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Suspend cells by pipetting. |
| Cell Number on Passage | 1-2 x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
| Cell No. | JCRB0708 | Cell Name | |
|---|---|---|---|
| LOT No. | 04112011 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 - 1.5 x 10^5 cells/mL | Methods for Passages | Simple dilution |
| Doubling Time | approx. 26 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 62.5 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0708 | Cell Name | P3U1 |
|---|---|---|---|
| LOT No. | 091097 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF001). | Temperature | 37 C |
| Cell Density at Seeding | 4x10^4 cells/ml | Methods for Passages | Simple dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.8x10^6 |
| Viability at cell freezing (%) | 95.0 | Antibiotics Used | free |
| Passage Number | P3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Under examination. |
| Chromosome Mode | NT | Chromosome Information | Under examination. |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0708 | Cell Name | |
|---|---|---|---|
| LOT No. | 073185 | Lot Specification | distribution |
| Medium | RPMI1640+10%FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | Suspend cells by pipetting. | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0708 | Cell Name | P3U1 |
|---|---|---|---|
| LOT No. | 05222017 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^5 cells/mL | Methods for Passages | Simple dilution. Some cells are adherent, and suspend cells by pipetting. |
| Doubling Time | approx. 18 hrs. | Cell Number in Vial (cells/1ml) | 2.8 x 10^6 |
| Viability at cell freezing (%) | 78.0 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Y/N | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 78.0 | Additional information |