JCRB0709.1 L5178Y TK+/-3.7.2c

Cell information

Important Notice(s)

Growth property of L5178Y TK+/-

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0709.1 Cell Name   L5178Y TK+/-3.7.2c
Profile   Mouse TK+/- heterozygotic subline of the L5178Y. Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   lymphoma induced by methylcholanthrene treatment Metastasis   
Tissue Metastasized    Genetics   leukemia
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   TK+/- heterozygotic subline of the L5178Y
Classify   transformed Established by   
Registered by    Regulation for Distribution   No regulation was applied to this strain to estab]
Comment   Incomplete description. Year   2002
Medium   Fischer's medium with 10% horse serum (original description). Current medium for propagation is RPMI 1640 medium with 10% fetal bovine serum. Methods for Passages   Suspend cells by pipetting.
Cell Number on Passage   2-3 x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6665
Reference
Pubmed id:3876312Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells.
Beer JZ,Mencl J,Horng MF,Gregg EC,Evans HH
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Oct;48(4):609-19
Pubmed id:6883332Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells.
Beer JZ,Budzicka E,Niepokojczycka E,Rosiek O,Szumiel I,Walicka M
Cancer Res. 1983 Oct;43(10):4736-42

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LOT Information

Cell No.   JCRB0709.1 Cell Name   L5178Y TK+/-3.7.2c
LOT No.   02052008 Lot Specification   distribution
Medium   Fischer's medium with 10% horse serum (heat-inactivation is recommended.) Temperature   37 C
Cell Density at Seeding   Suitable initial density is 2-3 x 10^4 cells/mL. Methods for Passages   Simple dilution (Suspension culture. Suspend the cells by pipetting)
Doubling Time   ~10 h (under RPMI+10%FBS) Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - Fischer's medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0709.1 Cell Name   L5178Y TK+/-3.7.2c
LOT No.   06182002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal calf serum(Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.5-3x10^4 cells/sq.cm. Methods for Passages   Simple dilution by pippeting. Suspension cell culture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.9 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0709.1 Cell Name   L5178Y TK+/-3.7.2c
LOT No.   04122021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding    Suitable initial density is 2-3 x 10^4 cells/mL. Methods for Passages   Simple dilution (Suspension culture. Suspend the cells by pipetting)
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^7
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   79 Additional information   
Cell No.   JCRB0709.1 Cell Name   L5178Y TK+/-3.7.2c
LOT No.   10152021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   Suitable initial density is 2-3 x 10^4 cells/mL. Methods for Passages    Simple dilution (Suspension culture. Suspend the cells by pipetting)
Doubling Time    Cell Number in Vial (cells/1ml)   7.6 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
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