JCRB0719 FSthy21
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB0719 | Cell Name | FSthy21 |
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Profile | complete defective strain of thymidylate synthase, (C3H mouse) | Other Name | FSthy21 |
Animal | mouse | Strain | C3H |
Genus | Mus | Species | musclus |
Sex | Age | ||
Identity | Tissue for Primary Cancer | thymidylate defective | |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | complete defective strain of thymidylate synthase | |
Life Span | Crisis PDL | ||
Morphology | Character | complete defective strain of thymidylate synthase | |
Classify | Established by | Ayusawa,D. | |
Registered by | Seno,K. | Regulation for Distribution | |
Comment | Thymidylate synthase,which is a target of 5-fluorouracil, 5-fluorodeoxyuridene,methotrexate, is completely deleted in this strain. | Year | |
Medium | ES medium ({3051}) or Eagle's minimal essetial medium with 2% fetal bovine serum and 2x10^-5M thymidine. | Methods for Passages | dilution twice a week, |
Cell Number on Passage | 10^5 cells/60mm dish | Race | |
CO2 Conc. | 5 % | Tissue Sampling | thymidylate defectiv |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:7105014 | Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate. Koyama H,Kodama H Cancer Res. 1982 Oct;42(10):4210-4 |