JCRB0719 FSthy21

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0719 Cell Name   FSthy21
Profile   complete defective strain of thymidylate synthase, (C3H mouse) Other Name   FSthy21
Animal   mouse Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   thymidylate defective
Case history    Metastasis   
Tissue Metastasized    Genetics   complete defective strain of thymidylate synthase
Life Span    Crisis PDL   
Morphology    Character   complete defective strain of thymidylate synthase
Classify    Established by   Ayusawa,D.
Registered by   Seno,K. Regulation for Distribution   
Comment   Thymidylate synthase,which is a target of 5-fluorouracil, 5-fluorodeoxyuridene,methotrexate, is completely deleted in this strain. Year   
Medium   ES medium ({3051}) or Eagle's minimal essetial medium with 2% fetal bovine serum and 2x10^-5M thymidine. Methods for Passages   dilution twice a week,
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   thymidylate defectiv
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_C564
Reference
Pubmed id:7105014Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate.
Koyama H,Kodama H
Cancer Res. 1982 Oct;42(10):4210-4

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