JCRB0737 Ad12-3Y1-Z19

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0737 Cell Name   Ad12-3Y1-Z19
Profile   3Y1 derivative, Ad12-transformed, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   whole embryo, Ad12-transformed 3Y1 subline
Life Span    Crisis PDL   
Morphology    Character   whole embryo, Ad12-transformed 3Y1 subline
Classify   new Established by   Kimura,G.
Registered by    Regulation for Distribution   Citation of #966 is required.
Comment   Prepare many ampoules when received cells and use new stock after 1-2 months continuous sub-culture. Year   1988
Medium   Dulbecco's modified Eagle's medium with 0.1%glucose and 10% fetal calf serum. Methods for Passages   0.05% trypsin & 5mM EDTA. Dilute 1/10. Prepare many frozen ampules when received.
Cell Number on Passage   Less than 1x10^7 cells/50mm D Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetal whole body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_F283
Reference
Pubmed id:6713382Imidazole-resistant phenotype and virus transformation in cultured rat cells.
Ikezaki K,Akiyama S,Miyazaki C,Kimura G,Kuwano M
Cancer Res. 1984 May;44(5):1791-5

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LOT Information

Cell No.   JCRB0737 Cell Name   Ad12-3Y1-Z19
LOT No.   012099 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Biocell laboratories, lot.4002171). Temperature   37 C
Cell Density at Seeding   3.5 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.05% trypsin and 5mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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