JCRB0814 VMRC-LCD

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0814 Cell Name   VMRC-LCD
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history    Metastasis   
Tissue Metastasized    Genetics   adenocarcinoma
Life Span    Crisis PDL   
Morphology    Character   adenocarcinoma
Classify    Established by   Akiyama,M.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1787
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 713869
Reference
Pubmed id:1752082The spread of human lung cancer cells on collagens and its inhibition by type III collagen.
Hirai K,Shimada H,Ogawa T,Taji S
Clin Exp Metastasis. 1991 Nov-Dec;9(6):517-27

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LOT Information

Cell No.   JCRB0814 Cell Name   
LOT No.   01182010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 51 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0814 Cell Name   VMRC-LCD
LOT No.   082598 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Summit, lot#8.1E+41). Temperature   37 C
Cell Density at Seeding   2x10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every four days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   P31 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LD, NP. Human cells not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:12
D7S820:8,10
D16S539:11
VWA:15,17
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0814 Cell Name   
LOT No.   081895 Lot Specification   distribution
Medium   MEM + 10%FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0814 Cell Name   VMRC-LCD
LOT No.   04272021 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) 10% heat inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   3.4-7.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.02x10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   p32 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:12
D7S820:8,10
D16S539:11
VWA:15,17
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   91.4 Additional information   
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