JCRB0819 SBC-5

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0819 Cell Name   SBC-5
Profile   small cell carcinoma, intermediate Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history    Metastasis   
Tissue Metastasized    Genetics   small cell carcinoma (intermediate)
Life Span    Crisis PDL   
Morphology    Character   small cell carcinoma (intermediate)
Classify    Established by   Hiraki,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1679
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 713880
Reference
Pubmed id:1330284In vitro measurement of chemosensitivity of human small cell lung and gastric cancer cell lines toward cell cycle phase-nonspecific agents under the clinically equivalent area under the curve.
Mitsuhashi Y,Inaba M,Sugiyama Y,Kobayashi T
Cancer. 1992 Nov 15;70(10):2540-6

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LOT Information

Cell No.   JCRB0819 Cell Name   
LOT No.   08082007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (lot:GIBCO484375) Temperature   37C
Cell Density at Seeding   1.5 x 10^5cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   21hr Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   90% Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   comfirmed as human by G6PD (type B), NP, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0819 Cell Name   
LOT No.   111898 Lot Specification   distribution
Medium   Eagle's minimal essential medium + 10% fetal calf serum (Biowhitttaker 6M0121) Temperature   37 C
Cell Density at Seeding   7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 40hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   92% Antibiotics Used   free
Passage Number   P39 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD(type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0819 Cell Name   
LOT No.   090895 Lot Specification   distribution
Medium   MEM + 10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P37 (BM3) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0819 Cell Name   
LOT No.   082895 Lot Specification   distribution
Medium   MEM + 10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicilline/streptomycin
Passage Number   P35 (BM3) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0819 Cell Name   SBC-5
LOT No.   09142018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.6 - 1.9 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
Cell No.   JCRB0819 Cell Name   SBC-5
LOT No.   11252020 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with 10% heat inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.62-4.42x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.33x10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   p39 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,10
D7S820:8,11
D16S539:12
VWA:14,18
TH01:6
AM:X,Y
TPOX:9,12
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   90.7 Additional information   
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