JCRB0821 NUGC-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0821 Cell Name   NUGC-2
Profile   adenocarcinoma, poorly differentiated Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   56
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   gastric cancer
Life Span    Crisis PDL   
Morphology    Character   gastric cancer
Classify    Established by   Akiyama,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 15% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/1 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1611
Reference
Pubmed id:2121682Alloimmunization with cultured human stomach cancer cell lines and the establishment of human-human hybridomas producing monoclonal antibodies.
Koyama K,Akiyama K,Kawahara H,Egashira A,Murakami H
Jpn J Cancer Res. 1990 Oct;81(10):967-70
Pubmed id:3172586Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4.
Akiyama S,Amo H,Watanabe T,Matsuyama M,Sakamoto J,Imaizumi M,Ichihashi H,Kondo T,Takagi H
Jpn J Surg. 1988 Jul;18(4):438-46

Images



LOT Information

Cell No.   JCRB0821 Cell Name   
LOT No.   07132009 Lot Specification   distribution
Medium   RPMI 1640 medium with 15% fetal bovine serum (GIBCO cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 -5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2.5 days Cell Number in Vial (cells/1ml)   8.3 x 10^5
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   NUGC-2
LOT No.   060888 Lot Specification   distribution
Medium   RPMI1640+10% FCS+2mM l-Gln+4mM HEPES+0.05%NaHCO3 Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.8x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P4 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   
LOT No.   090495 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P44 (MC210) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   
LOT No.   091195 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P45 (MC210) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   
LOT No.   091895 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P43 (MC210) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   
LOT No.   120297 Lot Specification   distribution
Medium   RPMI1640 medium with 15% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   3.9 x 10^5
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0821 Cell Name   NUGC-2
LOT No.   04282014 Lot Specification   distribution
Medium   RPMI 1640 medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   7.0 x 10^5
Viability at cell freezing (%)   87.5 Antibiotics Used   free
Passage Number   P53 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:8,11
D7S820:11
D16S539:7,12
VWA:17,18
TH01:7
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87.5 Additional information   
CLOSE