JCRB0822 NUGC-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0822 Cell Name   NUGC-3
Profile   adenocarcinoma, poorly differentiated Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   72
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   gastric cancer
Life Span    Crisis PDL   
Morphology    Character   gastric cancer
Classify    Established by   Akiyama,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and dilute into fresh medium. Change medium twice a week.
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1612
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908455
Reference
Pubmed id:2121682Alloimmunization with cultured human stomach cancer cell lines and the establishment of human-human hybridomas producing monoclonal antibodies.
Koyama K,Akiyama K,Kawahara H,Egashira A,Murakami H
Jpn J Cancer Res. 1990 Oct;81(10):967-70
Pubmed id:3172586Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4.
Akiyama S,Amo H,Watanabe T,Matsuyama M,Sakamoto J,Imaizumi M,Ichihashi H,Kondo T,Takagi H
Jpn J Surg. 1988 Jul;18(4):438-46

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LOT Information

Cell No.   JCRB0822 Cell Name   
LOT No.   11042011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0822 Cell Name   
LOT No.   04272009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   28 - 45 hrs. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   P39 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0822 Cell Name   
LOT No.   06062005 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.5 - 1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 26 hrs Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   120396 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi Kagaku, PVF01) Temperature   37 C
Cell Density at Seeding   1-1.5x10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 10 min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P37 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0822 Cell Name   
LOT No.   090195 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   02262016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   
Cell Density at Seeding   0.8 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   11162017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.9 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96.5 Additional information   Lot# is mislabeled as 11062017.
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   11072019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   4.0 - 8.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   01172022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.9 - 1.2 x 10^5 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   32 - 56 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   89.5 Additional information   
Cell No.   JCRB0822 Cell Name   NUGC-3
LOT No.   08042023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437. Lot 1072322) Temperature   37C
Cell Density at Seeding   0.7 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 - 40 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:10,11
D7S820:8,9
D16S539:10,11
VWA:18
TH01:6,8
AM:X
TPOX:8,9
CSF1PO:13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97.0 Additional information   
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