JCRB0826 8505C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0826 Cell Name   8505C
Profile   thyroid carcinoma, undifferentiated Other Name   zero-go-see
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   78
Identity   available Tissue for Primary Cancer   thyroid
Case history   undifferentiated thyroid carcinoma Metastasis   No
Tissue Metastasized    Genetics   C:G->G:C transversion at codon 248 of p53 gene
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Nakamura,N.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1988
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Cell Number on Passage   1.5x10^5 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   thyroid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1054
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 924102
Reference
Pubmed id:21566963Establishment of 2 human thyroid-carcinoma cell-lines (8305c, 8505c) bearing p53 gene-mutations.
Ito T,Seyama T,Hayashi Y,Hayashi T,Dohi K,Mizuno T,Iwamoto K,Tsuyama N,Nakamura N,Akiyama M
Int J Oncol. 1994 Mar;4(3):583-6
Pubmed id:1737400Unique association of p53 mutations with undifferentiated but not with differentiated carcinomas of the thyroid gland.
Ito T,Seyama T,Mizuno T,Tsuyama N,Hayashi T,Hayashi Y,Dohi K,Nakamura N,Akiyama M
Cancer Res. 1992 Mar 1;52(5):1369-71

Images



LOT Information

Cell No.   JCRB0826 Cell Name   8505C
LOT No.   03242008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305) Temperature   37 C
Cell Density at Seeding   1.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   NT
Passage Number   p29 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:13
D7S820:10
D16S539:12
VWA:17,19
TH01:6,9
AM:X
TPOX:11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0826 Cell Name   8505C
LOT No.   11252003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   6-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 1.5 days Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98% Additional information   
Cell No.   JCRB0826 Cell Name   
LOT No.   081595 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   >90 Antibiotics Used   
Passage Number   P23 PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0826 Cell Name   8505C
LOT No.   10192023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 174112) Temperature   37 C
Cell Density at Seeding   6.0 - 9.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:13
D7S820:10
D16S539:12
VWA:17,19
TH01:6,9
AM:X
TPOX:11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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