JCRB1017 HKA-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1017 | Cell Name | HKA-1 |
|---|---|---|---|
| Profile | A cell line derived from a keratoacanthoma of human skin, grow in a monolayer with anaplastic feature. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 77 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | a adome-shaped tumor(3cm) with a keratotic lump on the right cheek. | Metastasis | |
| Tissue Metastasized | Genetics | Chromosome is hypertriploidy with modal number of 78. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | A giant keratoacanthoma. Cells grow in a monolayer and are epithelioid with anaplastic feature. |
| Classify | tumor | Established by | Hozumi,Y. |
| Registered by | Hozumi,Y. | Regulation for Distribution | |
| Comment | Mycoplasmas decontaminated in the JCRB cell bank (NIHS0251, lot.02152002). | Year | 2001 |
| Medium | Dulbecco's modified Eagle's medium with 20% fetal bovine serum. | Methods for Passages | Cells harvested after treatment with 0.02% EDTA and 0.05% trypsin. Subculture once a week. |
| Cell Number on Passage | Cell no. not known. | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:none | Establishment and characterization of a cell line (HKA-1) derived from a human skin keratoacanthoma. Hozumi,Y. Yamagata Med. J., 10: 15-24, 1992. |
| Images |
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LOT Information
| Cell No. | JCRB1017 | Cell Name | HKA-1 |
|---|---|---|---|
| LOT No. | 10222013 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10099). | Temperature | 37 C |
| Cell Density at Seeding | 8.5 - 9.6 x 10^4 cells/mL | Methods for Passages | Cells harvested after treatment with 0.02% EDTA and 0.05% trypsin. Subculture once a week. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 cells/1mL | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P101 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB),NP,LDH were examined. Human,not HeLa |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90.5 | Additional information |