JCRB1019 RERF-LC-Sq1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1019 Cell Name   RERF-LC-Sq1
Profile   Cell line established from human lung carcinoma tissue. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   74
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer, squamous carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Established from human lung squamous carcinoma tissue grown in SCID mice.
Classify   tumor Established by   Teraoka,S. & Kyoizumi,S. et.al.
Registered by   Kyoizumi,S. Regulation for Distribution   not defined
Comment    Year   2001
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes. Subculture once in two weeks.
Cell Number on Passage   split ratio = 1:4 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1656
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298538
Reference
Pubmed id:21559605Scid mice model for the in-vivo study of human oncotherapy - studies on the growth and metastasis of human lung-cancer.
Teraoka S,Kyoizumi S,Seyama T,Yamakido M,Akiyama M
Int J Oncol. 1994 Sep;5(3):501-8

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LOT Information

Cell No.   JCRB1019 Cell Name   
LOT No.   09022008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 7 x 10^4 cells/mL Methods for Passages   0.05% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subcluture once in 1-2 week.
Doubling Time   very slow (D.T.=4-5 days) Cell Number in Vial (cells/1ml)   4.8 x 10^5
Viability at cell freezing (%)   82.4 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1019 Cell Name   RERF-LC-Sq1
LOT No.   07162015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.8-5.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.15x10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   p24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:11,12
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1019 Cell Name   RERF-LC-Sq1
LOT No.   08222017 Lot Specification   distribution
Medium    RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding    1.64-2.16x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.32x10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   p24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:11,12
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
Images
Cell No.   JCRB1019 Cell Name   RERF-LC-Sq1
LOT No.   08312017 Lot Specification   distribution2
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.64-2.16x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.34x10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   p25 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:11,12
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   79 Additional information   
Cell No.   JCRB1019 Cell Name   RERF-LC-Sq1
LOT No.   03272023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   2.4 - 4.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 90 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:11,12
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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