-Detailed Information [JCRB1025]-

JCRB1025 MKN7

Cell information

Important Notice(s)

Comment to the description of MKN7 cell line in "Human Cell Culture, Vol II"

-- Comment to the description of MKN7 cells in "Human Cell Culture, Vol II" --

In the following litarature, the MKN7 cell line is introduced as a mislabeled cell line.

Suzuki, T. and Sekiguchi, M. (1999). Gastric cancer. In: Human Cell Culture Vol II, Masters JRW & Palsson, B, eds., Kluwer Academic Publishers, Dordrecht, pp257-291.

However, this description is only the case report of mislabeling. The true MKN7 strain exists, and the MKN7 cell line supplied to users from JCRB / HSRRB cell bank is not mislabeled cell line.

In fact, JCRB cell bank received MKN7 cell line for two times. JCRB0082 MKN7 in 1986 and JCRB1025 MKN7 in 1989.

The first MKN7 strain was deposited by Dr. Suzuki (one of the author in the book) in 1986, and was assigned the registry number JCRB0082. The deposited JCRB0082 grow in suspension and was different from original description of MKN7. As in the book, the JCRB0082 MKN7 was apparently misidentified strain, and the JCRB cell bank deleted the registry of JCRB0082 immediately. This strain has not been provided to users.

The MKN7 referred in the book corresponds to the above JCRB0082.

The second deposition of MKN7 to JCRB cell bank was in 1989 by Dr. Hojo, the developer of this cell line. This strain was registered as JCRB1025. This strain is adherent culture and unique in STR profile. The all MKN7 cell line supplied to users is this JCRB1025 strain.



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JCRB No.	JCRB1025	Cell Name	MKN7
Profile	tubular adenocarcinoma, well differentiated	Other Name	MKN7
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	39-year-old
Identity	available	Tissue for Primary Cancer	stomach
Case history	stomach cancer	Metastasis
Tissue Metastasized		Genetics	stomach, well differentiated tubular adenocarcinoma
Life Span	infinite	Crisis PDL
Morphology		Character	stomach,well differentiated tubular adenocarcinoma
Classify	tumor	Established by	Hojo,H.
Registered by	Motoyama & Watanabe	Regulation for Distribution
Comment	39 year-old, male. Mycoplasma-eliminated cells by MC-210.	Year	1989
Medium	RPMI1640 medium with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage	split ratio=1/5	Race
CO2 Conc.	5 %	Tissue Sampling	stomach
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1417

Reference
Pubmed id:2736210	Production of transforming growth factor-alpha in human tumour cell lines. Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K Br J Cancer. 1989 May;59(5):761-5
Pubmed id:3095279	Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel. Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:3962675	Comparison of seven cell lines derived from human gastric carcinomas. Motoyama T,Hojo H,Watanabe H Acta Pathol Jpn. 1986 Jan;36(1):65-83

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LOT Information

08242012
02012011
06252009
07062007
03242003
08092018
08192020
06052023
04112023

Cell No.	JCRB1025	Cell Name
LOT No.	08242012	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	3.3 - 4.9 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 53 hrs	Cell Number in Vial (cells/1ml)	9.7 x 10^5 cells/mL
Viability at cell freezing (%)	92.9	Antibiotics Used	free
Passage Number	Unknown (>30)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1025	Cell Name
LOT No.	02012011	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2.2 - 2.7 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	57 - 87 hrs.	Cell Number in Vial (cells/1ml)	7.4 x 10^5
Viability at cell freezing (%)	81.3	Antibiotics Used	free
Passage Number	Unknown (29 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1025	Cell Name
LOT No.	06252009	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	50 - 70 hrs	Cell Number in Vial (cells/1ml)	1.0 x 10^6
Viability at cell freezing (%)	73.4	Antibiotics Used	free
Passage Number	Unknown (>29)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1025	Cell Name
LOT No.	07062007	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375)	Temperature	37 C
Cell Density at Seeding	2-3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	5.8 x 10^5
Viability at cell freezing (%)	81.5	Antibiotics Used	free
Passage Number	Unknown ( >P34)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1025	Cell Name
LOT No.	03242003	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F)	Temperature	37 C
Cell Density at Seeding	1 x 10^5 cells/ml.	Methods for Passages	0.25% trypsin and 0.02% EDTA for 10-15 min at 37 C
Doubling Time		Cell Number in Vial (cells/1ml)	1.0 X 10^6
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	>P31	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS in RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1025	Cell Name	MKN7
LOT No.	08092018	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	2.9 - 4.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 53 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	92.6	Antibiotics Used	free
Passage Number	Unknown (28 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	88.8	Additional information

Cell No.	JCRB1025	Cell Name	MKN7
LOT No.	08192020	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% ftal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	3.7 - 7.2 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 10 min at 37 C.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	94.9	Antibiotics Used	free
Passage Number	Unknown (29 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	97.4	Additional information

Cell No.	JCRB1025	Cell Name	MKN7
LOT No.	06052023	Lot Specification	distribution
Medium	RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,　Cat#172012,　Lot#12J396)	Temperature	37 C
Cell Density at Seeding	0.444-2.18x10^4 cells/cm2	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA for 10 minutes at 37 C.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.52x10^6
Viability at cell freezing (%)	99.4	Antibiotics Used	free
Passage Number	p30*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:9,13 D13S317:8,12 D7S820:9 D16S539:9,10 VWA:17 TH01:7,9 AM:X TPOX:9 CSF1PO:10,13
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	87.8	Additional information

Images

Cell No.	JCRB1025	Cell Name	MKN7
LOT No.	04112023	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322)	Temperature	37 C
Cell Density at Seeding	1.0 - 4.3 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	6.0 x 10^5
Viability at cell freezing (%)	99.2	Antibiotics Used	free
Passage Number	Unknown (>29)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9,13 D13S317:8,12 D7S820:9 D16S539:9,10 VWA:17 TH01:7,9 AM:X TPOX:9 CSF1PO:10,13
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	99.2	Additional information