JCRB1040 FF101
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1040 | Cell Name | FF101 |
|---|---|---|---|
| Profile | Rat hepatoma cell line with producing unidentified growth factor maintained in serum-free RPMI1640 medium for 444 passages. | Other Name | |
| Animal | rat | Strain | donryu |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | adult | |
| Identity | Tissue for Primary Cancer | liver | |
| Case history | hepatoma was induced by feeding 0.06% 3'-methyl-4-dimethylaminoazobenzene for five months. | Metastasis | |
| Tissue Metastasized | Genetics | Cells have characteristics of hepatoma and cholangioma. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | The cells are expected to produce FF-GF (unidentified growth factor, 70KDa). Sup. of the cell culture have growth activating activity(AH66, K562, Y3, YAC-1,BALB/c,3T3,NS-1). Cells have been cultured with serum-free medium from the primary cuture. |
| Classify | tumor | Established by | Matsumoto,H.et.al. |
| Registered by | Matsumoto,H | Regulation for Distribution | not defined |
| Comment | Cells have been maintained by continuous cell culture without freezing at all. | Year | 2002 |
| Medium | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). | Methods for Passages | Adhesion to dishes a weak. Pipetting with 2-3 times is enough to harvest cells. Subculture once every 7-10 days. |
| Cell Number on Passage | not defined. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1281996 | Purification and characterization of a novel growth factor (FF-GF) synthesized by a rat hepatoma cell line, FF101. Matsuda H,Matsumoto M,Kitahara H,Haraguchi S,Kanai K Biochem Biophys Res Commun. 1992 Dec 15;189(2):654-61 |
| Pubmed id:2702652 | Partial purification of a growth factor synthesized by a rat hepatoma cell line established in serum-free medium. Matsuda H,Matsumoto M,Haraguchi S,Kanai K Cancer Res. 1989 Apr 15;49(8):2118-22 |
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LOT Information
| Cell No. | JCRB1040 | Cell Name | FF101 |
|---|---|---|---|
| LOT No. | 10282003 | Lot Specification | distribution |
| Medium | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). | Temperature | 37C |
| Cell Density at Seeding | 3.4 x 10^4 cells/sq.cm | Methods for Passages | Pipetting with 2-3 times is enough to harvest cells. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 86.2 | Antibiotics Used | Free |
| Passage Number | P455 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium + FM-1 (1 to 1 mix) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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