JCRB1050 OVMIU-II

Cell information

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Problem of JCRB1047 OVSAYO、JCRB1049 OVMIU、JCRB1050 OVMIU-II

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1050 Cell Name   OVMIU-II
Profile   Human cell line derived from ovarian tumour. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   46
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian carcinoma, stage IIIc, CBDCA + TXL(4) treatment done before. Metastasis   No
Tissue Metastasized   recurrent tumor Genetics   tumour markers, CA125, ALP.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Experiments on mouse transplantation have not been done.
Classify   tumour Established by   Gorai,I. et.al.
Registered by   Gorai,I. Regulation for Distribution   
Comment   The STR pattern was unique while the pattern is expected to be same with the OVMIU. Year   2002
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage   5 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   reccurrent forcus
Tissue Type   adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3113
Reference
Pubmed id:11121861Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96

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LOT Information

Cell No.   JCRB1050 Cell Name   OVMIU-II
LOT No.   08092010 Lot Specification   distribution
Medium   RPMI1640 medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.55-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.55x10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   free
Passage Number   p47 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:14
D7S820:13,14
D16S539:11
VWA:14
TH01:9
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1050 Cell Name   OVMIU-II
LOT No.   05012003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P43 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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