JCRB1067 CPT-K5
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1067 | Cell Name | CPT-K5 |
|---|---|---|---|
| Profile | Mutant cell line derived from human ALL cell line, RPMI8402. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | Camptothecin resistant cell line. Purified DNA topoisomerase I was resistant to the camptothecin. |
| Classify | mutant | Established by | Kusunoki,Y. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | |
| Comment | Year | 2003 | |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum | Methods for Passages | Simple dilution. Subculture twice a week. |
| Cell Number on Passage | split ratio = 1/2 - 1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | |
| Tissue Type | mutant cell line from human ALL, RPMI8402 |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2831213 | Characterization of a camptothecin-resistant human DNA topoisomerase I. Kjeldsen E,Bonven BJ,Andoh T,Ishii K,Okada K,Bolund L,Westergaard O J Biol Chem. 1988 Mar 15;263(8):3912-6 |
| Pubmed id:3039492 | Characterization of a mammalian mutant with a camptothecin-resistant DNA topoisomerase I. Andoh T,Ishii K,Suzuki Y,Ikegami Y,Kusunoki Y,Takemoto Y,Okada K Proc Natl Acad Sci U S A. 1987 Aug;84(16):5565-9 |
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LOT Information
| Cell No. | JCRB1067 | Cell Name | CPT-K5 |
|---|---|---|---|
| LOT No. | 07282003 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum. | Temperature | 37 C |
| Cell Density at Seeding | 2.0 x 10^5 cells/ml | Methods for Passages | simple dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 98.9 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12,13 D13S317:10,12 D7S820:9,12,14 D16S539:10,14 VWA:18,20,22 TH01:6,7 AM:X TPOX:8 CSF1PO:9,10,11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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