-Detailed Information [JCRB1074]-

JCRB1074 FU97

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1074	Cell Name	FU97
Profile	Human stomach cancer cell line with AFP production.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	66
Identity	available	Tissue for Primary Cancer	stomach
Case history	stomach cancer, 2266ng/ml AFP observed before the operation. Lymph node metastasis and pancreatic metastasis observed. ss, ssINFbehta, ly3, v0.	Metastasis	Yes
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Td = 95 hrs. AFP production and tumorigenesity is maintained in the cell line.
Classify	tumor	Established by	Matsuda,M.
Registered by	Iwai,M.	Regulation for Distribution
Comment		Year	2003
Medium	Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal calf serum and 10mg/L insulin.	Methods for Passages	Cells are harvested after treatment with trypsin-EDTA.
Cell Number on Passage		Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	stomach
Tissue Type	poorly differentiated adenocarcinoma.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2908
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1290806

Reference
Pubmed id:10614861	Establishment of an alpha-fetoprotein-producing cell line derived from gastric cancer. Matsuda M,Watanabe A,Sawada H,Yamada Y,Nakano H,Iwai M,Iwai Y In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):555-7
Pubmed id:none	Biological behavior of an alpha-fetoprotein-producing gastric cancer cell line (FU97) Matsuda, M. J. Nara Med. Ass., 51: 79-89 (2000)

Images

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LOT Information

02022011
08072007
02052004
03132015
02192019
06122023

Cell No.	JCRB1074	Cell Name	FU97
LOT No.	02022011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (high glucose; 4.5 g/L) with 10 mg/L insulin and 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.4 - 2.2 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 34 hrs.	Cell Number in Vial (cells/1ml)	9.3 x 10^5
Viability at cell freezing (%)	73.8	Antibiotics Used	free
Passage Number	Unknown (12 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1074	Cell Name
LOT No.	08072007	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal calf serum (lot:GIBCO484375) and 10mg/L insulin.	Temperature	37C
Cell Density at Seeding	2.0 x 10^4cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in PBS
Doubling Time	30hr	Cell Number in Vial (cells/1ml)	7.5 x 10^6
Viability at cell freezing (%)	85	Antibiotics Used	free
Passage Number	P12*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by NP, G6PD (type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	70% Culture medium + 20% FCS + 10% DMSO	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1074	Cell Name	FU97
LOT No.	02052004	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (High glucose) with 10% fetal calf serum (Intergen RB52305) and 10mg/L insulin.	Temperature	37 C
Cell Density at Seeding	8.8 x 10^3 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.3% trypsin. Subculture once 5-7 days and medium change every 2-3 days.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	97.9	Antibiotics Used	free
Passage Number	P9*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5 % DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB1074	Cell Name	FU97
LOT No.	03132015	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) and 10 mg/L insulin.	Temperature	37 C
Cell Density at Seeding	1.3 - 1.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 31 hrs.	Cell Number in Vial (cells/1ml)	3.6 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (typeB), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,12 D13S317:10,12 D7S820:11 D16S539:9,11 VWA:17 TH01:9 AM:X TPOX:12 CSF1PO:12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	95	Additional information

Cell No.	JCRB1074	Cell Name	FU97
LOT No.	02192019	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) and 10 mg/L insulin	Temperature	37 C
Cell Density at Seeding	2.0 - 2.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 41 hrs.	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	90.3	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM (high glucose)	CO2 Conc.	5%
Viability immediately after thawing (%)	90.3	Additional information

Cell No.	JCRB1074	Cell Name	FU97
LOT No.	06122023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322)	Temperature	37 C
Cell Density at Seeding	1.8 - 2.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 36 hrs.	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	93	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,12 D13S317:10,12 D7S820:11 D16S539:9,11 VWA:17 TH01:9 AM:X TPOX:12 CSF1PO:12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM (high glucose)	CO2 Conc.	5%
Viability immediately after thawing (%)	83	Additional information