JCRB1148 KMBC-2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1148 Cell Name   KMBC-2
Profile   ******* Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   bladder
Case history    Metastasis   
Tissue Metastasized    Genetics   ******
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Doubling time for about 60 hours. A round cell proliferates like stripes. Because vacuoles are recognizable in the cytoplasm, secretion production is suggested.
Classify   tumor Established by   Shuin,T.
Registered by   Shuin,T. Regulation for Distribution   
Comment    Year   2005
Medium   Ham F12 medium with 10% Fetal Bovine Serum Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every one week at split ratio 1:9.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   urinary bladder
Virus Summary   tested [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
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LOT Information

Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   03292006 Lot Specification   distribution
Medium   Ham's F12 medium with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   8.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability (%)   94.8 Antibiotics Used   Free
Passage Number   P8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,10
D7S820:12
D16S539:9,11
VWA:18
TH01:8,9
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Additional information   
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Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   02082017 Lot Specification   distribution
Medium   Ham' F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   6.2 - 8.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 68 hrs. Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability (%)   85.4 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Additional information   
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