JCRB1179 KMS-11

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1179 Cell Name   KMS-11
Profile   human myeloma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   67
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   pleural effusion formation Metastasis   Yes
Tissue Metastasized    Genetics   t(4;14)(p16.3;q32.3)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Otsuki, T.
Registered by   Otsuki, T. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   Subculture twice a week by dilution.
Cell Number on Passage   50000 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   myeloma pleural effusion infiltration (IgGk)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2989
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1659817
Reference
Pubmed id:2004018Analysis of methylation in the c-MYC gene in five human myeloma cell lines.
Ohtsuki T,Nishitani K,Hatamochi A,Yawata Y,Namba M
Br J Haematol. 1991 Feb;77(2):172-9
Pubmed id:2519219[Establishment and characterization of five human myeloma cell lines].
Ohtsuki T,Yawata Y,Namba M
Hum Cell. 1989 Sep;2(3):297-303

Images



LOT Information

Cell No.   JCRB1179 Cell Name   
LOT No.   10142009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Adherent and non-adherant cells exist. Collect both types of cells (the adherent cells were harvested by scraping, pipetting, or trypsin treatment), and resuspend to fresh medium.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   78.6 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   +/- Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1179 Cell Name   
LOT No.   03062008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat.#10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1.5 - 5 x 10^5 cells/ml Methods for Passages   Most cells were non-adherant but a portion of cells were adherent. Collect both types of cells (the latters were harvested by scraping or pipetting). Subculture twice a week.
Doubling Time   50 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   88.9 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1179 Cell Name   KMS-11
LOT No.   11252006 Lot Specification   distribution
Medium   RPMI1640 with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   9.7x10^4 cells/sq.cm Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:11,12
D16S539:9,13
VWA:17,18
TH01:6,9
AM:X
TPOX:12
CSF1PO:13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1179 Cell Name   KMS-11
LOT No.   08022013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% featal bovine serum (FBS; GIBCO Cat. #10099). Temperature   37C
Cell Density at Seeding   3-4 x 10^5 cells / mL Methods for Passages   Adherent and non-adherent cells exist. Collect both types of cells (the adherent cells were harvested by scraping, pipetting, or trypsin treatment), and resuspend to fresh medium.
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, MD were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   79.4% Additional information   
Cell No.   JCRB1179 Cell Name   KMS-11
LOT No.   10072016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% featal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.4 - 4.0 x 10^5 cells/mL Methods for Passages   Adherent and non-adherent cells exist. Collect both types of cells (the adherent cells were harvested by scraping, pipetting, or trypsin treatment), and resuspend to fresh medium.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   82.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   +/- Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   82.5 Additional information   
Cell No.   JCRB1179 Cell Name   KMS-11
LOT No.   04092018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/mL Methods for Passages   Both of adherent and non-adherent cells exist. (The adherent cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA or Accutase.)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.81x10^6
Viability at cell freezing (%)   80.7 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:11,12
D16S539:9,13
VWA:17,18
TH01:6,9
AM:X
TPOX:12
CSF1PO:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB1179 Cell Name   KMS-11
LOT No.   10222021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.1 - 2.1 x 10^5 cells/mL Methods for Passages   Adherent and non-adherent cells exist. Collect both types of cells (the adherent cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA), and resuspend to fresh medium.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   6.8 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.4 Additional information   
CLOSE