JCRB1180 KMM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1180 Cell Name   KMM-1
Profile   human myeloma cell line Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   No
Tissue Metastasized    Genetics   t(8;14)(q24;q32), t(6;14)(p25;q32)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Togawa, A.
Registered by   Otsuki, T Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   Subculture twice a week by dilution(1/10).
Cell Number on Passage   50000 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   subcutaneous tumor
Tissue Type   myeloma (Igλ)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2981
Reference
Pubmed id:2004018Analysis of methylation in the c-MYC gene in five human myeloma cell lines.
Ohtsuki T,Nishitani K,Hatamochi A,Yawata Y,Namba M
Br J Haematol. 1991 Feb;77(2):172-9
Pubmed id:7095894Establishment and characterization of a human myeloma cell line (KMM-1).
Togawa A,Inoue N,Miyamoto K,Hyodo H,Namba M
Int J Cancer. 1982 May 15;29(5):495-500

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LOT Information

Cell No.   JCRB1180 Cell Name   
LOT No.   07032012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.2 - 1.7 x 10^5 Methods for Passages   This cell line is mixture of adherent and non-adherent cells. Recover both non-adherent cells and adherent cells. The adherent cells were detached by pipetting or 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 47 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   86.3 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1180 Cell Name   KMM-1
LOT No.   12192006 Lot Specification   distribution2
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2.4x10^5 cells/ml Methods for Passages   Subculture twice a week by dilution(1/10).
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.2x10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1180 Cell Name   KMM-1
LOT No.   11282006 Lot Specification   distribution
Medium   RPMI1640 with 10% heat inactivated fetal bovine serum(Hyclone ALA12955) Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   82.9 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,12
D7S820:9,10
D16S539:11
VWA:16,17
TH01:6
AM:X
TPOX:9,11
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1180 Cell Name   KMM-1
LOT No.   07142017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% featal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.1 - 1.4 x 10^5 cells/mL Methods for Passages   This cell line is mixture of adherent and non-adherent cells. Recover both non-adherent cells and adherent cells. The adherent cells were detached by pipetting or 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94.7 Additional information   
Cell No.   JCRB1180 Cell Name   KMM-1
LOT No.   04212021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.7 - 5.2 x 105 cells/mL Methods for Passages   This cell line is mixture of adherent and non-adherent cells. Recover both non-adherent cells and adherent cells. The adherent cells were detached by pipetting or 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx .24 hrs Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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