JCRB1208 HM-1 Cell information
Cell type:general cells
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JCRB No.
JCRB1208
Cell Name
HM-1
Profile
Hamster macrophage cell line from Syrian hamster transplanted with human umbilical cord leukocytes.
Other Name
Animal
hamster, Syrian
Strain
Syrian
Genus
Mesocricetus
Species
auratus
Sex
Age
Identity
not done
Tissue for Primary Cancer
hemo-lymphocytic
Case history
human umbilical cord leukocytes had been transplanted
Metastasis
Tissue Metastasized
Genetics
hamster cell
Life Span
infinite
Crisis PDL
Morphology
lymphocyte-like
Character
macrophage
Classify
normal
Established by
Miyoshi,I.
Registered by
Miyoshi,I.
Regulation for Distribution
Comment
Cell growth is slow. When cell growth becomes faster, please check a characeteristics of the cell.
Year
2007
Medium
RPMI 1640 medium with 10 % fetal calf serum
Methods for Passages
dilution
Cell Number on Passage
1/2 - 1/3
Race
CO2 Conc.
5%
Tissue Sampling
lymph node
Tissue Type
enlarged axillary lymph node
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:657122 Establishment and characterization of two hamster macrophage cell lines. Miyoshi I,Hiraki S,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Masuji H,Kimura I Cancer Lett. 1978 May;4(5):253-7
LOT Information
Cell No.
JCRB1208
Cell Name
HM-1
LOT No.
09032007
Lot Specification
distribution
Medium
RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051)
Temperature
37 C
Cell Density at Seeding
3.2x10^5 cells/ml
Methods for Passages
subculture by dilution
Doubling Time
NT
Cell Number in Vial (cells/1ml)
5.2x10^6
Viability at cell freezing (%)
92.3
Antibiotics Used
free
Passage Number
p5*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
No
Exoteric Gene
NT
Medium for Freezing
Cell Banker BLC-1(Nihon Zenyaku Industries)
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information