JCRB1222.02 HGH1.6.5
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1222.02 | Cell Name | HGH1.6.5 |
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Profile | Mouse hybridoma cell line producing monoclonal antibody against human growth hormone (hGH). | Other Name | |
Animal | mouse | Strain | |
Genus | hybridoma | Species | |
Sex | Age | ||
Identity | not done | Tissue for Primary Cancer | antibody-producing hybridoma |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | lymphocyte-like | Character | Producing IgG gamma2a, and k. |
Classify | hybridoma | Established by | Sawada, J. |
Registered by | Sawada, J. | Regulation for Distribution | |
Comment | Year | 2007 | |
Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | dilution |
Cell Number on Passage | split 1/5 (1/2 - 1/10) | Race | |
CO2 Conc. | 5% | Tissue Sampling | |
Tissue Type | producing Mab human growth hormone (hGH). |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:2427940 | Comparison of the antigenicities of native human growth hormone (hGH) and three forms of recombinant hGHs using monoclonal antibodies. Sawada J,Wada N,Irie M,Tokunaga-Doi T,Ohtsuka E,Ikehara M,Terao T Mol Immunol. 1986 Jun;23(6):625-30 |
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LOT Information
Cell No. | JCRB1222.02 | Cell Name | HGH1.6.5 |
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LOT No. | 07142008 | Lot Specification | distribution |
Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
Cell Density at Seeding | Cell Banker BLC-1(Nihon Z | Methods for Passages | split 1/5 (1/2-1/10) |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.7x10^6 |
Viability at cell freezing (%) | 79.8 | Antibiotics Used | free |
Passage Number | p5* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | |
Adhesion | No | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
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