JCRB1223 NCC-RbC-T1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1223 | Cell Name | NCC-RbC-T1 |
|---|---|---|---|
| Profile | Human retinoblastoma cell line. The growth is very slow. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 1.5 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | Bilateral retinoblastoma metastasis | Metastasis | Yes |
| Tissue Metastasized | Genetics | Bilateral retinoblastoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | |
| Classify | tumor | Established by | Inomata, M. |
| Registered by | Inomata, M. | Regulation for Distribution | Not applied. |
| Comment | Year | 2006 | |
| Medium | CMRL1066 medium with 10-15% fetal bovine serum (100 μM Glutamine, 200 μM Sodium pyruvate and 20 μM 2-mercaptoetanol) | Methods for Passages | Simple dilution. split ratio=1/5. Subculture every 1 week. |
| Cell Number on Passage | 8 x 10^5 cells / mL | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | Metastasis in cerebrospinal fluid |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8263010 | Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol. Inomata M,Kaneko A,Saijo N,Tokura S J Cancer Res Clin Oncol. 1994;120(3):149-55 |
| Pubmed id:3115934 | Chemosensitivity profiles of primary and cultured human retinoblastoma cells in a human tumor clonogenic assay. Inomata M,Kaneko A Jpn J Cancer Res. 1987 Aug;78(8):858-68 |
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LOT Information
| Cell No. | JCRB1223 | Cell Name | NCC-RbC-T1 |
|---|---|---|---|
| LOT No. | 01172008 | Lot Specification | distribution |
| Medium | CMRL1066 medium(GIBCO) with 100uM Glutamine ,200uM Sodium pyruvate,50uM 2-Mercaptoetanol and 20% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 5.9x10^5 cells/ml | Methods for Passages | Simple dilution to 1:2 split per week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9x10^6 |
| Viability at cell freezing (%) | 65.7 | Antibiotics Used | NT |
| Passage Number | p11* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:11 D7S820:9,10 D16S539:11 VWA:14 TH01:7,8 AM:X,Y TPOX:8,10 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1223 | Cell Name | NCC-RbC-T1 |
|---|---|---|---|
| LOT No. | 03172014 | Lot Specification | distribution |
| Medium | CMRL1066 medium with 100 μM glutamine, 200 μM sodium pyruvate, 20 μM 2-mercaptoetanol, and 20% FBS (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | approx. 5 x 10^5 cells/mL | Methods for Passages | Simple dilution. 1:2 split per week. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.3 x 10^6 | |
| Viability at cell freezing (%) | 85.1 | Antibiotics Used | free |
| Passage Number | Unknown (14 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,13 D13S317:11 D7S820:9,10 D16S539:11 VWA:14 TH01:7,8 AM:X,Y TPOX:8,10 CSF1PO:12 |
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| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 85.1 | Additional information |
| Cell No. | JCRB1223 | Cell Name | NCC-RbC-T1 |
|---|---|---|---|
| LOT No. | 06212023 | Lot Specification | distribution |
| Medium | CMRL1066 medium with 100 μM glutamine, 200 μM sodium pyruvate, 50 μM 2-mercaptoetanol, and 15% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.5-3.0x10^5/ml | Methods for Passages | Dilution (split ratio=1/2) Subculture every one week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.0x10^6 |
| Viability at cell freezing (%) | 86.2 | Antibiotics Used | free |
| Passage Number | p14* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:11 D7S820:9,10 D16S539:11 VWA:14 TH01:7,8 AM:X,Y TPOX:8,10 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | uncountable | Additional information |
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