JCRB1223 NCC-RbC-T1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1223 Cell Name   NCC-RbC-T1
Profile   Human retinoblastoma cell line. The growth is very slow. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   1.5
Identity   available Tissue for Primary Cancer   neural
Case history   Bilateral retinoblastoma metastasis Metastasis   Yes
Tissue Metastasized    Genetics   Bilateral retinoblastoma
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   
Classify   tumor Established by   Inomata, M.
Registered by   Inomata, M. Regulation for Distribution   Not applied.
Comment    Year   2006
Medium   CMRL1066 medium with 10-15% fetal bovine serum (100 μM Glutamine, 200 μM Sodium pyruvate and 20 μM 2-mercaptoetanol) Methods for Passages   Simple dilution. split ratio=1/5. Subculture every 1 week.
Cell Number on Passage   8 x 10^5 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   Metastasis in cerebrospinal fluid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3054
Reference
Pubmed id:8263010Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol.
Inomata M,Kaneko A,Saijo N,Tokura S
J Cancer Res Clin Oncol. 1994;120(3):149-55
Pubmed id:3115934Chemosensitivity profiles of primary and cultured human retinoblastoma cells in a human tumor clonogenic assay.
Inomata M,Kaneko A
Jpn J Cancer Res. 1987 Aug;78(8):858-68

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LOT Information

Cell No.   JCRB1223 Cell Name   NCC-RbC-T1
LOT No.   01172008 Lot Specification   distribution
Medium   CMRL1066 medium(GIBCO) with 100uM Glutamine ,200uM Sodium pyruvate,50uM 2-Mercaptoetanol and 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   5.9x10^5 cells/ml Methods for Passages   Simple dilution to 1:2 split per week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   65.7 Antibiotics Used   NT
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:9,10
D16S539:11
VWA:14
TH01:7,8
AM:X,Y
TPOX:8,10
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1223 Cell Name   NCC-RbC-T1
LOT No.   03172014 Lot Specification   distribution
Medium   CMRL1066 medium with 100 μM glutamine, 200 μM sodium pyruvate, 20 μM 2-mercaptoetanol, and 20% FBS (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^5 cells/mL Methods for Passages   Simple dilution. 1:2 split per week.
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   85.1 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:9,10
D16S539:11
VWA:14
TH01:7,8
AM:X,Y
TPOX:8,10
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   85.1 Additional information   
Cell No.   JCRB1223 Cell Name   NCC-RbC-T1
LOT No.   06212023 Lot Specification   distribution
Medium   CMRL1066 medium with 100 μM glutamine, 200 μM sodium pyruvate, 50 μM 2-mercaptoetanol, and 15% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   2.5-3.0x10^5/ml Methods for Passages   Dilution (split ratio=1/2) Subculture every one week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   86.2 Antibiotics Used   free
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:9,10
D16S539:11
VWA:14
TH01:7,8
AM:X,Y
TPOX:8,10
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   uncountable Additional information   
Images
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