JCRB1304 MT-45-5-3
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1304 | Cell Name | MT-45-5-3 |
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Profile | Mouse hybridoma cell line producing monoclonal antibody against metallothionein. | Other Name | |
Animal | mouse | Strain | |
Genus | hybridoma | Species | |
Sex | Age | ||
Identity | not done | Tissue for Primary Cancer | antibody-producing hybridoma |
Case history | Metastasis | No | |
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | lymphocyte-like | Character | Producing IgG1(K) |
Classify | hybridoma | Established by | Kikuchi, Y. |
Registered by | kikuchi, Y. | Regulation for Distribution | |
Comment | Year | 2007 | |
Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. | Methods for Passages | dilution |
Cell Number on Passage | split 1/5 (1/2 - 1/10) | Race | |
CO2 Conc. | 5% | Tissue Sampling | |
Tissue Type | producing Mab metallothionein. |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:1500065 | Identification of metallothionein in cultured cells by immunoblotting and immunofluorescence using a new monoclonal antibody. Kikuchi Y,Irie M,Ikebuchi H,Sawada J,Terao T Hybridoma. 1992 Jun;11(3):295-300 |
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LOT Information
Cell No. | JCRB1304 | Cell Name | MT-45-5-3 |
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LOT No. | 03282008 | Lot Specification | distribution |
Medium | DMEM with 10% heat inactivated fetal bovine serum(Intergen Lot.RB52305) | Temperature | 37 C |
Cell Density at Seeding | 6.9x10^4 cells/ml | Methods for Passages | dilution 1/5(1/2-1/10) |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.2x10^6 |
Viability at cell freezing (%) | 88.0 | Antibiotics Used | NT |
Passage Number | p5* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | |
Adhesion | No | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
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