JCRB1343 OKa-C-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1343 | Cell Name | OKa-C-1 |
|---|---|---|---|
| Profile | Lung cancer cell line producing both G-CSF and PTHrP. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 64 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | Poorly differentiated squamous cell carcinoma with leukocytosis and hypercalcemia. | Metastasis | |
| Tissue Metastasized | Genetics | Chromosome numbers are 75 and 78 with complex structural abnormality. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | The cell line was established from the transplanted Balb 3T3 mouse. The cell line have a characteristics of inducing leukocytosis and hypercalcemia with high G-CSF and PTHrP levels. Td=95 hrs. Adherent to the culture flasks. |
| Classify | tumor | Established by | Kubonishi, I. |
| Registered by | Miyoshi, I. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum. | Methods for Passages | Cells are harvested after treatment with trypsin and EDTA. Scraping may be OK. |
| Cell Number on Passage | Split ratio = 1/2 - 1/3. | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | tumor in the left lung at autopsy, |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8641981 | Establishment of a clonal cell line producing granulocyte colony-stimulating factor and parathyroid hormone-related protein from a lung cancer patient with leukocytosis and hypercalcemia. Asahi Y,Kubonishi I,Imamura J,Kamioka M,Matsushita H,Furihata M,Ohtsuki Y,Miyoshi I Jpn J Cancer Res. 1996 May;87(5):451-8 |
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LOT Information
| Cell No. | JCRB1343 | Cell Name | OKa-C-1 |
|---|---|---|---|
| LOT No. | 03172010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 15% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-2.8x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA and split ratio=1/2-1/3 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.8x10^6 |
| Viability at cell freezing (%) | 95.5 | Antibiotics Used | NT |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1343 | Cell Name | OKa-C-1 |
|---|---|---|---|
| LOT No. | 06022023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat#11875-093) with 20% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.89-3.94x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.22x10^6 |
| Viability at cell freezing (%) | 96.62 | Antibiotics Used | free |
| Passage Number | p16* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:8 D7S820:10,11 D16S539:10 VWA:14,18 TH01:6,9 AM:X,Y TPOX:8,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94 | Additional information |
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