JCRB1349 Ex-3LL

Cell information

Important Notice(s)

Lewis lung carcinoma (3LL): Culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1349 Cell Name   Ex-3LL
Profile   Variant cell line possessing enhanced lung metastasis ability derived from Lewis lung cancer (3LL). Other Name   
Animal   mouse Strain   --
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   lung
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Lewis lung carcinoma derived cell line enhanced lung metastasis ability.
Classify   tumor Established by   Ota, T
Registered by   Ota, T Regulation for Distribution   
Comment    Year   2008
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation: RPMI-1640 medium with 10% FBS) Methods for Passages   Cells harvested after treatment with PBS containing 0.125% trypsin and 1 mM EDTA. Change medium twice a week.
Cell Number on Passage   -- Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5748
Reference
Pubmed id:11299736Functional suppression of integrin beta 4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation.
Ota T,Maeda M,Tanino M,Tatsuka M
Anticancer Res. 2001 Jan-Feb;21(1A):205-11
Pubmed id:10408901Decrease of metastatic ability after selection for intravasating ability in Lewis lung carcinoma (3LL) cell line.
Ota T,Maeda M,Tatsuka M,Matsui T,Tanino M,Tanaka T
Cancer Lett. 1999 May 3;139(1):105-8

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LOT Information

Cell No.   JCRB1349 Cell Name   Ex-3LL
LOT No.   11132009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with PBS containing 0.125% trypsin and 1mM EDTA. Change medium twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1349 Cell Name   Ex-3LL
LOT No.   08052015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091). Temperature   37 C
Cell Density at Seeding   1.0 - 2.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs Cell Number in Vial (cells/1ml)   5.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD , MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Cell No.   JCRB1349 Cell Name   Ex-3LL
LOT No.   10282020 Lot Specification   distribution
Medium   RPMI 1640 medium with heat-inactivated fetal bovine serum (FBS; Biowest Cat. # S18200-500) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   4.5 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
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