JCRB1351 Int-3LL

Cell information

Important Notice(s)

Lewis lung carcinoma (3LL): Culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1351 Cell Name   Int-3LL
Profile   Variant cell line possessing enhanced intravasating ability derived from Lewis lung cancer (3LL). Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   lung
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Lewis lung carcinoma derived cell line enhanced intravasating ability.
Classify   tumor Established by   Ota, T
Registered by   Ota, T Regulation for Distribution   
Comment    Year   2008
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation: RPMI-1640 medium with 10% FBS) Methods for Passages   Cells were harvested with pipetting.
Cell Number on Passage   -- Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8358
Reference
Pubmed id:11299736Functional suppression of integrin beta 4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation.
Ota T,Maeda M,Tanino M,Tatsuka M
Anticancer Res. 2001 Jan-Feb;21(1A):205-11
Pubmed id:10408901Decrease of metastatic ability after selection for intravasating ability in Lewis lung carcinoma (3LL) cell line.
Ota T,Maeda M,Tatsuka M,Matsui T,Tanino M,Tanaka T
Cancer Lett. 1999 May 3;139(1):105-8

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LOT Information

Cell No.   JCRB1351 Cell Name   Int-3LL
LOT No.   11162009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.1-4.3x10^4 cells/ml Methods for Passages   Cells were harvested with pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.5x10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1351 Cell Name   Int-3LL
LOT No.   02142017 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.6 - 2.2 x 10^5 cells/mL Methods for Passages   Cells were harvested by pipetting.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   76.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   76.7 Additional information   
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