JCRB1352 EL-4ad

Cell information

Important Notice(s)

EL-4 and EL-4ad: Culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1352 Cell Name   EL-4ad
Profile   A valiant cell line which adhered to a tissue culture dish was isolated from highly metastatic EL-4 murine T-lymphoma. Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   adhered to a tissue culture dish
Classify   tumor Established by   Ota, T.
Registered by   Ota, T. Regulation for Distribution   
Comment    Year   2008
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation: RPMI-1640 medium with 10% FBS) Methods for Passages   Cell were harvested with 0.02% EDTA.
Cell Number on Passage   -- Race   
CO2 Conc.   5% Tissue Sampling   T-lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8603
Reference
Pubmed id:1505120Isolation and characterization of a low metastatic variant from EL-4 mouse T-lymphoma.
Ota T,Tanino M,Kohno H,Funamoto H,Odashima S
Clin Exp Metastasis. 1992 Sep;10(5):297-308

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LOT Information

Cell No.   JCRB1352 Cell Name   EL-4ad
LOT No.   11202009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.0-9.0x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1352 Cell Name   EL-4ad
LOT No.   06062018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.9 - 4.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   85.7 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85.7 Additional information   
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