JCRB1413 ST 13
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1413 | Cell Name | ST 13 |
|---|---|---|---|
| Profile | Mouse normal preadipocyte cell line established from ddN mouse. ST 13 can be induced to differentiate into fat-accumulating cells in vitro. | Other Name | |
| Animal | mouse | Strain | ddN |
| Genus | Mus | Species | musculus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | differentiate to adipocyte | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | normal | Established by | Sato, M. |
| Registered by | Sato, M. | Regulation for Distribution | |
| Comment | Year | 2010 | |
| Medium | HAM:DMEM(1:1) medium with 10 % fetal calf serum | Methods for Passages | 0.02% EDTA and 0.25% Trypsin. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | mammary gland |
| Tissue Type | normal |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
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| Pubmed id:16857754 | Calpain system regulates the differentiation of adult primitive mesenchymal ST-13 adipocytes. Yajima Y,Sato M,Sorimachi H,Inomata M,Maki M,Kawashima S Endocrinology. 2006 Oct;147(10):4811-9 |
| Pubmed id:12746319 | Mechanism of adult primitive mesenchymal ST-13 preadipocyte differentiation. Yajima Y,Sato M,Sumida M,Kawashima S Endocrinology. 2003 Jun;144(6):2559-65 |
| Pubmed id:11824563 | Dehydrotrametenolic acid induces preadipocyte differentiation and sensitizes animal models of noninsulin-dependent diabetes mellitus to insulin. Sato M,Tai T,Nunoura Y,Yajima Y,Kawashima S,Tanaka K Biol Pharm Bull. 2002 Jan;25(1):81-6 |
| Pubmed id:3275678 | Preadipocyte differentiation in vitro: identification of a highly active adipogenic agent. Hiragun A,Sato M,Mitsui H J Cell Physiol. 1988 Jan;134(1):124-30 |
| Pubmed id:2982429 | Differentiation-associated increase of cAMP-dependent type II protein kinase in a murine preadipose cell line (ST 13). Sato M,Hiragun A,Mitsui H Biochim Biophys Acta. 1985 Mar 21;844(3):296-305 |
| Pubmed id:6998485 | Preadipocytes possess cellular retinoid binding proteins and their differentiation is inhibited by retinoids. Sato M,Hiragun A,Mitsui H Biochem Biophys Res Commun. 1980 Aug 29;95(4):1839-45 |
| Pubmed id:6998859 | Establishment of a clonal cell line that differentiates into adipose cells in vitro. Hiragun A,Sato M,Mitsui H In Vitro. 1980 Aug;16(8):685-93 |
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LOT Information
| Cell No. | JCRB1413 | Cell Name | ST 13 |
|---|---|---|---|
| LOT No. | 02162012 | Lot Specification | distribution |
| Medium | DMEM/ F12(1/1) medium(GIBCO 11320-033) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.53-2.1x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.06x10^6 |
| Viability at cell freezing (%) | 96.8 | Antibiotics Used | |
| Passage Number | p14* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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