JCRB1415 KasumiA-568

Cell information

Important Notice(s)

Procedure for addition of GM-CSF

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1415 Cell Name   KasumiA-568
Profile   myeloblastic leukemia Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   65
Identity   available Tissue for Primary Cancer   
Case history   acute myeloid leukemia (FABM6) Metastasis   
Tissue Metastasized    Genetics   45,XY,del(2)(q?),-13,-16,-19,add(19)(q13.1),add(21)(q22),+mar1,+mar2
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   CD13+, CD33+, c-kit+, GPA+
Classify   tumor Established by   Asou, H.
Registered by   Asou, H Regulation for Distribution   
Comment    Year   2009
Medium   RPMI1640 medium with 20% featal bovine serum and GM-CSF (10ng/mL). Methods for Passages   dilution (split ratio 1:2)
Cell Number on Passage   0.5-2.0 x 10^6 cells Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8525
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LOT Information

Cell No.   JCRB1415 Cell Name   KasumiA-568
LOT No.   07042011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and 10ng/ml rhu GM-CSF. Temperature   37 C
Cell Density at Seeding   0.25-1.0x10^6 cells/ml Methods for Passages   Dilution (split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.60x10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,12
D7S820:12
D16S539:9,11
VWA:17
TH01:7
AM:X,Y
TPOX:8
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1415 Cell Name   KasumiA-568
LOT No.   10192018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) and GM-CSF (10ng/mL). Temperature   37 C
Cell Density at Seeding   2.0-3.0 x 10^5 cells Methods for Passages   dilution (split ratio 1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,12
D7S820:12
D16S539:9,11
VWA:17
TH01:7,9
AM:X,Y
TPOX:8
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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