JCRB1475 Nalm-6-MSH+

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1475 Cell Name   Nalm-6-MSH+
Profile   Human cell line derived from B cell leukemia. (MSH+, POL(WT), TK+/-) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19-year-old
Identity   available Tissue for Primary Cancer   
Case history   acute lymphoblastic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   MSH+, POL(WT), TK+/-
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   
Registered by   Nohmi, T. Regulation for Distribution   Depositor's approval is required.
Comment    Year   2012
Medium   RPMI 1640 + 10% heat-inactivate FBS Methods for Passages   dilution
Cell Number on Passage   twice/week, split ratio = 1:10 Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J365
Reference
Pubmed id:27338670Sensitivity of human cells expressing low-fidelity or weak-catalytic-activity variants of DNA polymerase ζ to genotoxic stresses.
Suzuki T,Grúz P,Honma M,Adachi N,Nohmi T
DNA Repair (Amst). 2016 Sep;45():34-43
Pubmed id:23596518Restoration of mismatch repair functions in human cell line Nalm-6, which has high efficiency for gene targeting.
Suzuki T,Ukai A,Honma M,Adachi N,Nohmi T
PLoS One. 2013;8(4):e61189

Images



LOT Information

Cell No.   JCRB1475 Cell Name   Nalm-6-MSH+
LOT No.   06012012 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.1-2.0x10^6 cells/ml Methods for Passages   Dilution(1/10),twice/week
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9x10^6
Viability at cell freezing (%)   85.9 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:9,13
D7S820:8,12
D16S539:9,11
VWA:15,16
TH01:8,9
AM:X,Y
TPOX:8,10
CSF1PO:14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1475 Cell Name   Nalm-6-MSH+
LOT No.   01192018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 1 day. Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   86 Additional information   
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