JCRB1503 NIH3T3/ATCC/CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1503 Cell Name   NIH3T3/ATCC/CMV-Luc
Profile   Luciferase stably expressing cell line (NIH/3T3; Mouse embryonic skin cell line). Other Name   
Animal   mouse Strain   NIH/Swiss
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   pLL3.7-luc transfected. Luciferase stably expressing cell line. Selection marker does not exist.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   DMEM medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   Inoculate 3 to 5 X 10(3) cells Race   
CO2 Conc.   5% Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y01
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LOT Information

Cell No.   JCRB1503 Cell Name   NIH3T3/ATCC/CMV-Luc
LOT No.   11292012 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.05-.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.47x10^6
Viability at cell freezing (%)   99.7 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Mouse confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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