JCRB1542 KP-3L-Luc#5

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

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Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1542 Cell Name   KP-3L-Luc#5
Profile   Luciferase stably expressing cell line (KP-3L; Human tumor cell line obtained from metastasis of adenosquamous carcinoma of the pancreatic duct). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75-year-old
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic tumor Metastasis   Yes
Tissue Metastasized    Genetics   pMSCV-luc transfected. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Tissue was classified as a metastasis of adenosquamous carcinoma of the pancreatic duct. Puromycin-resistant.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   1-2 x 10^5 cells/T25 frask Race   
CO2 Conc.   5% Tissue Sampling   KP-3L was established from the resu
Tissue Type   pancreas

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y22
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LOT Information

Cell No.   JCRB1542 Cell Name   KP-3L-Luc#5
LOT No.   06282013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.78-7.01x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.46x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:10,12
D16S539:9,13
VWA:18
TH01:6,9.3
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1542 Cell Name   KP-3L-Luc#5
LOT No.   06212024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.5 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   Passage number p8* printed on cryotube is incorrect. Correct passage number is p11*.
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