JCRB1545 EM-E6/E7/hTERT-2
Cell information
Important Notice(s)Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB1545 | Cell Name | EM-E6/E7/hTERT-2 |
|---|---|---|---|
| Profile | Immortalized human uterine endometril progenitor cell line. | Other Name | EPC-2 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Human uterine endometrial progenitor cell line immortalized by infection with recombinant retroviruses expressing the E6, E7, and hTERT. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | transformed | Established by | Kyo, S. et al. |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2011 | |
| Medium | Poweredby 10 | Methods for Passages | When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | uterine endometrium |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:17314403 | Menstrual blood-derived cells confer human dystrophin expression in the murine model of Duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation. Cui CH,Uyama T,Miyado K,Terai M,Kyo S,Kiyono T,Umezawa A Mol Biol Cell. 2007 May;18(5):1586-94 |
| Pubmed id:14633600 | Successful immortalization of endometrial glandular cells with normal structural and functional characteristics. Kyo S,Nakamura M,Kiyono T,Maida Y,Kanaya T,Tanaka M,Yatabe N,Inoue M Am J Pathol. 2003 Dec;163(6):2259-69 |
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LOT Information
| Cell No. | JCRB1545 | Cell Name | EM-E6/E7/hTERT-2 |
|---|---|---|---|
| LOT No. | 07172013 | Lot Specification | distribution |
| Medium | POWEREDBY10 | Temperature | 37 C |
| Cell Density at Seeding | 1.5-7.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.(Split ratio=1/2-1/5) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.74x10^6 |
| Viability at cell freezing (%) | 97.8 | Antibiotics Used | NT |
| Passage Number | p12 | PDL | 27.6 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:8 D7S820:11,12 D16S539:11,12 VWA:16,20 TH01:7,9 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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