JCRB1554 MMNK-1

Cell information

Important Notice(s)

This cell line is available for only academic users.

本細胞は学術機関へのみの分譲となります

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1554 Cell Name   MMNK-1
profile   Highly differentiated immortalized human cholangiocyte cell line. Alias   
animal   human strain   
genus   Homo species   sapiens
sex    age   
identity   available tissue   
case history    metastasis   No
metastorgn    genetics   Expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP).
lifespan   infinite crisispdl   
morphology   epithelial-like charact   
classify   transformed originator   Kobayashi, N.
register   Mochitate, K. regulation   [Maruyama et al. Transplantation 77: 446-451, 2004] should be cited at the paper submission.
comment   The distribution is limieted to academic Japan domestic users and academic oversea users. The following reference should be cited at the publication of paper. Maruyama et al. Transplantation 77: 446-451, 2004. dyear   2013
medium   DMEM with 5% fetal bovine serum pasmethod    Cells were harvested after treatment with Accutase and split ratio(1/3-1/4)
pascellno    race   
co2conc   5% tohroku   registered
preporgn    vrsummary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control]
vrsdetect   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) tissueform   cholangiocytes (OUMS-21)


Reference
Pubmed id:14966424 Establishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT.
Maruyama M,Kobayashi N,Westerman KA,Sakaguchi M,Allain JE,Totsugawa T,Okitsu T,Fukazawa T,Weber A,Stolz DB,Leboulch P,Tanaka N
Transplantation. 2004 Feb 15;77(3):446-51

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LOT Information

Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   09142013 LOTSPEC   distribution
MEDIUM   DMEM with 5% fetal bovine serum(SIGMA027K03911,non HI) GROWTEMP   37 C
PASCELLNO   0.4-1.5x10^5 cells/sq.cm PASMETHOD   Cells were harvested after treatment with Accutase and split ratio(1/3-1/4)
GROWTHRATE   NT FREEZCONC   2.15x10^6
VIABLFREEZ   97.0 ANTIBIOTIC   free
PASSAGE   p4* PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:11,15
D13S317:9,12
D7S820:8,9
D16S539:11,13
VWA:14,17
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,12
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2   5 %
RFLP   NT Additional information   


Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   02022017 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) GROWTEMP   37 C
PASCELLNO   5.3 - 6.3 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with Accutase.
GROWTHRATE   approx. 32 hrs FREEZCONC   1.2 x 10^6
VIABLFREEZ   84.9 ANTIBIOTIC   free
PASSAGE   Unknown (7 at bank) PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    Additional information   


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