JCRB1563 HAdpc-29-E7-TERT

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1563 Cell Name   HAdpc-29-E7-TERT
Profile   Immortalized human adipose tissue-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Human adipose tissue-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E7 and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Derived from subcutaneous adipose tissue around mammary gland. hTERT, HPV E7 genes were transfected.
Classify   transformed Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2011
Medium   Poweredby 10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   adipocyte

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W40
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LOT Information

Cell No.   JCRB1563 Cell Name   HAdpc-29-E7-TERT
LOT No.   11252013 Lot Specification   distribution
Medium   POWEREDBY10(within serum and biotics) Glyco Thechinica Temperature   37 C
Cell Density at Seeding   1.00-6.97x10^4cells/sq.cm Methods for Passages   When the cultures reached subconfluence,the cells were harvested after treatment with 0.25% trypsin and 1mM EDTA and replaced with one-half of harvested cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.13x10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   
Passage Number   p15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:11,12
D16S539:9,13
VWA:17,19
TH01:7
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1563 Cell Name   HAdpc-29-E7-TERT
LOT No.   06052024 Lot Specification   distribution
Medium   POWEREDBY10(within serum:emmukk) Temperature   37 C
Cell Density at Seeding   2.0-4.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   100.00 Antibiotics Used   free
Passage Number   p18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:11,12
D16S539:9,13
VWA:17,19
TH01:7
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   97.5 Additional information   
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