JCRB1571 F6B3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1571 Cell Name   F6B3
Profile   Mouse cell line derived from mouse brain tumor. Other Name   SR-C3H/St glioma
Animal   mouse Strain   C3H/St
Genus   Mus Species   musclus
Sex   - Age   -
Identity   not done Tissue for Primary Cancer   
Case history   - Metastasis   No
Tissue Metastasized    Genetics   SR-RSV-induced brain tumor cells
Life Span   infinite Crisis PDL   
Morphology   polygonal-fibroblast Character   GFAP (glial fibrillary acidic protein positive)
Classify   tumor Established by   Shimbo, Y.
Registered by   Kumanishi, T. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   0.02% Trypsin + 0.01% EDTA
Cell Number on Passage   1:3, 1 or 2 times/week Race   
CO2 Conc.    Tissue Sampling   brain
Tissue Type   brain tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y23
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LOT Information

Cell No.   JCRB1571 Cell Name   F6B3
LOT No.   02142014 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.77-2.85x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.01% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.41x10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   p41 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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