JCRB1633 HuH-6-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1633 Cell Name   HuH-6-Luc
Profile   Luciferase stably expressing cell line (HuH-6; Human Hepatoma Cell Line). Other Name   JTC38
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   infant
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   hepatoblastoma Metastasis   No
Tissue Metastasized    Genetics   pLVSIN-luc transfected HuH-6 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Produces albumin, prealbumin, fibrinogen etc. Puromycin-resistant.
Classify   tumor Established by   Doi,I. & Sato,J. -> Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2015
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Treat cells with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage   More than 5x10^4 cells/cm^2 Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JF84
Images
Movies



LOT Information

Cell No.   JCRB1633 Cell Name   HuH-6-Luc
LOT No.   07242015 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.18-1.7x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.21x10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   p9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:8,12
D7S820:11,12
D16S539:10,11
VWA:14,17
TH01:7,8
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)   75.76 Additional information   
Images
Cell No.   JCRB1633 Cell Name   HuH-6-Luc
LOT No.   07282023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322). Temperature   37 C
Cell Density at Seeding   0.7 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8,11
D13S317:8,12
D7S820:11,12
D16S539:10,11
VWA:14,17
TH01:7,8
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
CLOSE