JCRB1642 KMS-11/BTZ
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1642 | Cell Name | KMS-11/BTZ |
|---|---|---|---|
| Profile | bortezomib-resistant multiple myeloma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 67-year-old |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | pleural effusion formation | Metastasis | |
| Tissue Metastasized | Genetics | t(4;14)(p16.3;q32.3) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | small round-cell morphology, part is multinucleate cell. |
| Classify | tumor | Established by | Nakashima, T. |
| Registered by | Hasegawa, M. | Regulation for Distribution | |
| Comment | Year | 2015 | |
| Medium | RPMI1640 medium with 10% featal bovine serum. | Methods for Passages | Subculture twice a week by dilution. |
| Cell Number on Passage | 50000 cells / mL | Race | |
| CO2 Conc. | 5% | Tissue Sampling | pleural effusion |
| Tissue Type | myeloma pleural effusion infiltration (IgGk) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:20555361 | Bortezomib-resistant myeloma cell lines: a role for mutated PSMB5 in preventing the accumulation of unfolded proteins and fatal ER stress. Ri M,Iida S,Nakashima T,Miyazaki H,Mori F,Ito A,Inagaki A,Kusumoto S,Ishida T,Komatsu H,Shiotsu Y,Ueda R Leukemia. 2010 Aug;24(8):1506-12 |
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LOT Information
| Cell No. | JCRB1642 | Cell Name | KMS-11/BTZ |
|---|---|---|---|
| LOT No. | 09292015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-6.0x10^5 cells/ml | Methods for Passages | dilution,(1/2-1/6) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.10x10^6 |
| Viability at cell freezing (%) | 95.1 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:12 D7S820:11,12 D16S539:9,13 VWA:17,18 TH01:6,9 AM:X TPOX:12 CSF1PO:13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1642 | Cell Name | KMS-11/BTZ |
|---|---|---|---|
| LOT No. | 07092020 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 2.5 - 3.1 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | approx. 35 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 88.7 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 85.4 | Additional information |