JCRB1670 HT-1080/CMV-Luc
Cell information
Important Notice(s)発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1670 | Cell Name | HT-1080/CMV-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (HT-1080; Human Sarcoma Cell Line with activated N-ras oncogene). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 35-year-old |
| Identity | available | Tissue for Primary Cancer | sarcoma |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | The cells contain activated N-ras oncogene. pLVSIN-luc transfected HT-1080 cells. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | puromycin-resistant |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | To exchange and follow MTA between oversea end-user and cell-developer. |
| Comment | Year | 2016 | |
| Medium | Eagle's Minimal Essential medium with non -essential amino acids and 10% fetal calf serum. | Methods for Passages | Treat cells with 0.02% EDTA and 0.25% trypsin. |
| Cell Number on Passage | dilute 1/4 - 1/8 | Race | Caucasian |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | fibrosarcoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Movies |
|---|
| JCRB1670_04012016.flv |
LOT Information
| Cell No. | JCRB1670 | Cell Name | HT-1080/CMV-Luc |
|---|---|---|---|
| LOT No. | 04012016 | Lot Specification | distribution |
| Medium | MEM(GIBCO) with non-essential amino acids and 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.369-1.00x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.08x10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:12,14 D7S820:9,10 D16S539:9,12 VWA:14,19 TH01:6 AM:X,Y TPOX:8 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98 | Additional information |