JCRB1835 HeLa-ACE2-TMPRSS2

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1835 Cell Name   HeLa-ACE2-TMPRSS2
Profile   HeLa cells expressing angiotensin-converting enzyme-related carboxypeptidase (ACE2) and transmembrane serine protease TMPRSS2. Recommended use: Isolation of human coronavirus NL63 (HCoV-NL63) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31-year-old
Identity   not done Tissue for Primary Cancer   uterine cervix
Case history   carcinoma of cervix Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Shirato, Kazuya
Registered by   Kazuya Shirato Regulation for Distribution   
Comment    Year   
Medium   DMED+5%FCS+G418(1mg/ml)+PS(FUJIFILM WAKO Chemicals No.168-23191,etc.) Methods for Passages   Trypsin-EDTA(Kojin Bio Co.,Ltd :No.16208004, etc.)
Cell Number on Passage    Race   Black
CO2 Conc.   5% Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B3LV
Reference
Pubmed id:22496216Simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.
Kawase M,Shirato K,van der Hoek L,Taguchi F,Matsuyama S
J Virol. 2012 Jun;86(12):6537-45

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LOT Information

Cell No.   JCRB1835 Cell Name   HeLa-ACE2-TMPRSS2
LOT No.   12012020 Lot Specification   distribution
Medium   DMEM(GIBCO:catalog No.11885-084) with G-418(1mg/ml) and 5% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.1-2.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.89x10^6
Viability at cell freezing (%)   96.72 Antibiotics Used   G-418
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:13.3,14.3
D7S820:8,12,13
D16S539:9,10
VWA:16,18,19
TH01:7
AM:X
TPOX:8,12
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   68.9 Additional information   
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Cell No.   JCRB1835 Cell Name   HeLa-ACE2-TMPRSS2
LOT No.   04262024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 1 mg/mL G418 and 5% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   23-26 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   G418
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.5 Additional information   
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