JCRB1850 Cx43KOM104-E4(+/-)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1850 Cell Name   Cx43KOM104-E4(+/-)
Profile   Connexin 43 +/- mouse embryo-derived fibroblast cell line. (embryo from Connexin 43 +/- parents) Other Name   
Animal   mouse Strain   C57BL/6
Genus    Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Gja1 (+/-)
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Yamakage, K.
Registered by   Kohji Yamakage Regulation for Distribution   
Comment    Year   
Medium   DMEM medium 10% FBS. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B3M8
Reference
Pubmed id:9808166Growth control of 3T3 fibroblast cell lines established from connexin 43-deficient mice.
Yamakage K,Omori Y,Piccoli C,Yamasaki H
Mol Carcinog. 1998 Oct;23(2):121-8

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LOT Information

Cell No.   JCRB1850 Cell Name   Cx43KOM104-E4(+/-)
LOT No.   03012021 Lot Specification   distribution
Medium   DMEM(GIBCO：Cat No.;11885-084) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   3.3-7.6x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.15x10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   p94 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   85.0 Additional information   
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