JCRB1852 Cx43KOM104-E6(-/-)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1852 | Cell Name | Cx43KOM104-E6(-/-) |
|---|---|---|---|
| Profile | Connexin 43 -/- mouse embryo-derived fibroblast cell line. (embryo from Connexin 43 +/- parents) | Other Name | |
| Animal | mouse | Strain | C57BL/6 |
| Genus | Species | ||
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Gja1 (-/-) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | transformed | Established by | Yamakage, K. |
| Registered by | Kohji Yamakage | Regulation for Distribution | |
| Comment | Year | ||
| Medium | DMEM medium 10% FBS. | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | embryo, whole |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9808166 | Growth control of 3T3 fibroblast cell lines established from connexin 43-deficient mice. Yamakage K,Omori Y,Piccoli C,Yamasaki H Mol Carcinog. 1998 Oct;23(2):121-8 |
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LOT Information
| Cell No. | JCRB1852 | Cell Name | Cx43KOM104-E6(-/-) |
|---|---|---|---|
| LOT No. | 03192021 | Lot Specification | distribution |
| Medium | DMEM(GIBCO：Cat No.;11885-084) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 3.1-7.6x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.19x10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | p93 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 72.4 | Additional information |
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