JCRB1884 HSC-60
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1884 | Cell Name | HSC-60 |
|---|---|---|---|
| Profile | Human signet ring cell gastric carcinoma cell line establised in vitro from ascites. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 40-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | scirrhous stomach cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Adherent cells. |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin-0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15245593 | Establishment of two cell lines from human gastric scirrhous carcinoma that possess the potential to metastasize spontaneously in nude mice. Yanagihara K,Tanaka H,Takigahira M,Ino Y,Yamaguchi Y,Toge T,Sugano K,Hirohashi S Cancer Sci. 2004 Jul;95(7):575-82 |
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LOT Information
| Cell No. | JCRB1884 | Cell Name | HSC-60 |
|---|---|---|---|
| LOT No. | 02042022 | Lot Specification | distribution |
| Medium | RPMI1640 medium (GIBCO：Cat No.;11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012-500ML, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-5x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3-1/10) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.94x10^6 |
| Viability at cell freezing (%) | 96.5 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:9,11 D7S820:8,11 D16S539:9 VWA:17 TH01:9 AM:X TPOX:8 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 93.3 | Additional information |