JCRB1894 TCC-MESO-1
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1894 | Cell Name | TCC-MESO-1 |
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Profile | Cell line of malignant pleural mesothelioma. TCC-MESO-2 established from same individual. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 58-year-old |
Identity | available | Tissue for Primary Cancer | Pleura |
Case history | Pleural epithelioid mesothelioma | Metastasis | |
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | epithelial-like | Character | Doubling time: 24.5 hours, Adherent cells. |
Classify | tumor | Established by | Yanagihara Kazuyoshi |
Registered by | Yuki Iino | Regulation for Distribution | |
Comment | Year | ||
Medium | RPMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin- 0.02% EDTA |
Cell Number on Passage | split ratio=1/10 | Race | Japanese |
CO2 Conc. | 5% | Tissue Sampling | left pleura |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:19876922 | An orthotopic implantation mouse model of human malignant pleural mesothelioma for in vivo photon counting analysis and evaluation of the effect of S-1 therapy. Yanagihara K,Tsumuraya M,Takigahira M,Mihara K,Kubo T,Ohuchi K,Seyama T Int J Cancer. 2010 Jun 15;126(12):2835-46 |
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LOT Information
Cell No. | JCRB1894 | Cell Name | TCC-MESO-1 |
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LOT No. | 04152022 | Lot Specification | distribution |
Medium | RPMI1640 medium (GIBCO:Cat No.11875) with 10% heat inactivated fetal bovine serum(SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
Cell Density at Seeding | 1.40-2.48x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05%trypsin(GIBCO) and 0.02% EDTA. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.18x10^6 |
Viability at cell freezing (%) | 98 | Antibiotics Used | free |
Passage Number | p5* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:10,11 D13S317:9 D7S820:8,12 D16S539:10,11 VWA:17 TH01:7 AM:X,Y TPOX:8,9 CSF1PO:12,14 |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 96 | Additional information |
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