JCRB1932 57L9Luc
Cell information
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1932 | Cell Name | 57L9Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line. Liver metastasis cell line derived from gastric cancer (well differentiated adenocarcinoma: HSC-57) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 76-year-old |
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | gastric cancer (well differentiated adenocarcinoma) | Metastasis | Y |
| Tissue Metastasized | Liver | Genetics | Luciferase gene-introduced cell line with pEGFPLuc plasmid vector |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | adherent cells. |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 medium + 10% FBS + G418(400 ug/mL). Do not add G418 at thawing. | Methods for Passages | 0.05% Trypsin-0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | Liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:24483192 | Marked antitumor effect of NK012, a SN-38-incorporating micelle formulation, in a newly developed mouse model of liver metastasis resulting from gastric cancer. Yanagihara K,Takigahira M,Kubo T,Ochiya T,Hamaguchi T,Matsumura Y Ther Deliv. 2014 Feb;5(2):129-38 |
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LOT Information
| Cell No. | JCRB1932 | Cell Name | 57L9Luc |
|---|---|---|---|
| LOT No. | 02142023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) and G418(400 ug/ml) | Temperature | 37 C |
| Cell Density at Seeding | 0.27-1.5-x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA (Split ratio=1/3-1/6) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.1x10^6 |
| Viability at cell freezing (%) | 91.10 | Antibiotics Used | G418 |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,14 D13S317:8,9 D7S820:10,12 D16S539:10,12 VWA:14 TH01:7 AM:X TPOX:8,10 CSF1PO:12,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cells culture after thawing must start without G418. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 78.9 | Additional information |
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