JCRB1941 TU-KatoIII

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1941 Cell Name   TU-KatoIII
Profile   Cell line isolated as 100% tumor-forming cells after tumor formation in immunodeficient mice (NOD Scid→Scid→nu/nu). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57-year-old
Identity   available Tissue for Primary Cancer   stomach
Case history   Signet ring cell gastric adenocarcinoma. Derived from metastatic site: Pleural effusion. Metastasis   Y
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round shape Character   floating
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   RPMI1640 medium + 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   Pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:17505008AZD2171 shows potent antitumor activity against gastric cancer over-expressing fibroblast growth factor receptor 2/keratinocyte growth factor receptor.
Takeda M,Arao T,Yokote H,Komatsu T,Yanagihara K,Sasaki H,Yamada Y,Tamura T,Fukuoka K,Kimura H,Saijo N,Nishio K
Clin Cancer Res. 2007 May 15;13(10):3051-7

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LOT Information

Cell No.   JCRB1941 Cell Name   TU-KatoIII
LOT No.   04072023 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396).  Temperature   37 C
Cell Density at Seeding   0.6-5.7x10^5 cells/ml Methods for Passages   Dilution (split ratio=1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.6x10^6
Viability at cell freezing (%)   88.90 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,12
D7S820:8,12
D16S539:12
VWA:14,16
TH01:7
AM:X
TPOX:11
CSF1PO:7,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   80.1 Additional information   Most cells grow in suspension.
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