JCRB1944 SH101-P4
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1944 | Cell Name | SH101-P4 |
|---|---|---|---|
| Profile | Subclone of SH101 (Human Gastric Cancer) cell line. Well differenciated adenocarcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | Gastric tubular adenocarcinoma. Derived from metastatic site: Ascites. | Metastasis | Y |
| Tissue Metastasized | ascites | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Adherent cells. |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin-0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8242641 | Antiproliferative effects of isoflavones on human cancer cell lines established from the gastrointestinal tract. Yanagihara K,Ito A,Toge T,Numoto M Cancer Res. 1993 Dec 1;53(23):5815-21 |
| Pubmed id:8473041 | Relevance of DT-diaphorase activity to mitomycin C (MMC) efficacy on human cancer cells: differences in in vitro and in vivo systems. Nishiyama M,Saeki S,Aogi K,Hirabayashi N,Toge T Int J Cancer. 1993 Apr 1;53(6):1013-6 |
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LOT Information
| Cell No. | JCRB1944 | Cell Name | SH101-P4 |
|---|---|---|---|
| LOT No. | 04042023 | Lot Specification | distribution |
| Medium | RPMI1640 medium (GIBCO:catalog No.11875-093) with 10% heat inactivated fetal bovine serum (Biowest cat.No. S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 5.63-8.91x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.42x10^6 |
| Viability at cell freezing (%) | 99.3 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:8,12 D7S820:11,12 D16S539:9 VWA:16,17 TH01:7 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91.1 | Additional information |